Applications of DNA Based Biosensors: A Review

  • Vandana Tandasi Jaypee Institute of Information Technology, Sector-62, Noida, Uttar Pradesh, India


Biosensors are analytical devices used to detect the presence or concentration of a biological component, such as a biomolecule, a biological structure or a microorganism, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc. The present study explores various DNA-based biosensors with various detection techniques that helps in diagnosis of acute and chronic diseases based on hybridization with specific sequences of the probe used in the biosensor that has the advantage of easy synthesis of nucleotide sequence of the target analyte. The DNA biosensors have a lot of applications in environmental and medical fields, in genetically modified organism (GMO) detection, diagnosis of genetic diseases, cancer detection, diagnosis of neurological diseases, biosensing of toxicity and diagnosing of infectious diseases.


Abdulmalek Hassan, Lee Yook Heng, Ling Ling tan (2019) Novel DNA Biosensor for Direct Determination of Carrageenan. Scientific Reports 9:63-79

Arun RC (2017) DNA Nanobiosensors: An Outlook on Signal Readout Strategies, Hindawi J Nanomaterials 9.

Asal M, Özen Ö, ?ahinler M,Polato?lu ? (2018) Recent Developments in Enzyme, DNA and Immuno-Based Biosensors, Sensors (Basel). 13: 18(6). doi: 10.3390/s18061924

Bora U, Sett A, Sing D (2013) Nucleic Acid Based Biosensors for Clinical Applications. Biosens J 1. doi:10.4172/2090-4967.1000104

Cady NC, Strickland AD, Batt CA (2007) Optimized linkage and quenching strategies for quantum dot molecular beacons. Mol Cell Probes 21: 116-124

Debnath Mousumi, Prasad GBKS, Bisen PS (2010) Molecular Diagnostics: Promises and Possibilities Dordrech Heidelberg London, Springer, pp 209-226

Dyadyusha L, Yin H, Jaiswal S, Brown T, Baumber JJ, Booy FP, Melvin T (2005) Quenching of CdSe quantum dot emission, a new approach for biosensing. Chem Commun 7: 3201–3203

Francis D. Krampa (2017) Recent Progress in the Development of Diagnostic Tests for Malaria. Diagnostics 7(3): 54. doi:10.3390/diagnostics7030054

Gill R, Bahshi L, Freeman R, Willner I (2008) Optical detection of glucose and acetylcholine esterase inhibitors by H2O2-sensitive CdSe/ZnS quantum dots. Angew Chem Int Ed 47: 1676–1679

Han X, Gao JZ, Su S Nie (2001) Quantum-dot tagged microbeads for multiplexed optical coding of biomolecules, Nature Publishing Group 19: 631-635

Hansen J, Wang AN, Kawde Y, Xiang V, Gothelf G, Collins (2006) Quantum dot aptamer based ultrasensitive multi analyte electrochemical biosensor. J Am Chem Soc 128(7): 2228-2229

Kavita V (2017) DNA Biosensors-A Review. J Bioengineer Biomedical Sci 7(2). DOI: 10.4172/2155-9538.1000222.

Khalid M, Abu-Salah, Mohammed M, Zourob Fouzi Mouffouk, Salman A, Alrokayan Manal A, Alaamery, Anees A Ansari (2015) DNA-Based Nanobiosensors as an Emerging Platform for Detection of Disease. Sensors 15: 14539-14568. doi: 10.3390/s150614539

Lin CW, Shulok JR, Wong YK, Schanbacher CF, Cincotta L, Foley JW (1991) Photosensitization, uptake, and retention of phenoxazine Nile Blue derivatives in human bladder carcinoma cells. Cancer Res 51:1109-16

Liu X, Farmerie W, Schuster S, Tan W (2000) Molecular beacons for DNA biosensors with micrometer to submicrometer dimensions. Anal Biochem 283: 56-63

Meric B, Kerman K, Marrazza G, Palchetti I, Mascini M, Ozsoz M (2004) Disposable genosensor, a new tool for detection of NOS-terminator, a genetic element present in GMOs. Food Control 15:621-6.

Miyake Y, Togashi H, Tashiro M, Yamaguchi H, Oda S, Kudo M, Tanaka Y, Kondo Y, Sawa R, Fujimoto T et al. (2006) MercuryII - mediated Formation of Thyamine-HgII-Thyamine Base Pairs in DNA Duplexes. J Am Chem Soc 128: 2172–2173

Nasrin Sohrabi, Alireza Valizadeh, Samad Mussa Farkhani, Abolfazl Akbarzadeh (2014) Basics of DNA biosensors and cancer diagnosis. Artificial Cells 44(2). DOI: 10.3109/21691401.2014.976707

Rella R, Spadavecchia J, Manera MG, Sciliano P, Santino A, Mita G (2004) Biosens Bioelectron 20: 1140-1148

Rodovalho VR (2015) Biosensors Applied to Diagnosis of Infectious Diseases – An Update. Austin J Biosens Bioelectron 1(3): 10-15

Stobiecka M (2013) Novel DNA-Biosensors for Studies of GMO, Pesticides and Herbicides: Chapter -10, AgriState of the Art in Biosensors - Environmental and Medical Application: pp-201-222

Stobiecka M, Cie?la JM, Janowska B, Tudek B, RadeckaH (2007) Piezoelectric sensor for determination of genetically modified soybean Roundup Ready in samples not amplified by PCR. Sensors 7: 1462-79

Tanaka Y, Oda S, Yamaguchi H, Kondo Y, Kojima C, Ono A (2007) 15N-15NJ-Coupling Across HgII: Direct Observation of HgII-Mediated T-T Base Pairs in a DNA Duplex. J Am Chem Soc 129: 244–245

Tang B, Cao L, Xu K, Zhuo L, Ge J, Li Q, Yu L (2008) A new nano-biosensor for glucose with high sensitivity and selectivity in serum based on fluorescence resonance Energy transfer (FRET) between CdTe quantum dots and Au nanoparticles. Chemistry 14: 3637–3644

Taylar D, Yu Q, Chen S, Homola J, Jiang S (2005) Comparison of E. Coli, 157:H7 preparation methods used for detection with surface resonance sensor. Sens Actuators B 107: 202-208

Watts HJ, Yeung D, Parkes H (1995) Real time detection and quantification of DNA hybridization by an optical biosensor. Anal Chem 67: 4283-4289

Wang YM, Pang XF, Zhang YY (2009) Recent advances in fiber-optic DNA biosensors. J Biomed Sci Eng 2: 312–317

Weihong JL, Wang K, Xiao D, Yang X, He X, Tang Z (2001) Ultrasensitive optical DNA biosensor based on surface immobilization of molecular beacon by a bridge structure. Anal Sci 17: 1149-1153

Wang R, Tombelli S, Minunni M, Michelia M, Spiriti M Mascini (2004) Immobilization of DNA probes for the development of SPR- based sensing, Biosens. Bioelectron 20: 967-974

Wang Y, Evangelyn C Alocilja (2015) Gold nanoparticle-labeled biosensor for rapid and sensitive detection of bacterial pathogens. J Biological Engineering 9:16 DOI 10.1186/s13036-015-0014-z

Yu F, Yao D, Knoll W (2004) Oligonucleotide hybridization studied by a surface plasmon diffraction sensor (SPDS), Nucl Acid Res 32(9): 1-7

How to Cite
Tandasi, V. 2019. Applications of DNA Based Biosensors: A Review. Journal of Biological Sciences and Medicine. 5, 3 (Sep. 2019), 1-7.
Review Articles